Fig 1: Expression levels of the proteins in NCM460 cells co-cultured with HT29 cells at different glucose concentrations. (A) Protein bands of NT5DC3, p-PI3K, PI3K, p-AKT, AKT, p-mTOR, mTOR, TNF-a, and IL-1ß, determined via Western blotting. (B) Statistical analysis of the p-PI3K/PI3K, p-AKT/AKT, and p-mTOR/mTOR ratios. The data are presented as mean ± SD, * p < 0.05 compared with the control group. This experiment was repeated three times (n = 3).
Fig 2: The anti-tumor effect of lactoferrin (LF) and NT5DC3 in high glucose conditions. (A) Co-culturing model consisting of NCM460 cells and HT29 cells. HT29 cell growth culture medium was further utilized to cultivate the NCM460 cells. (B) Glucose concentration selection using the NCM460 cells and a cell viability assay. Here, 2–5 g/L of glucose was regarded as the appropriate concentration for subsequent experiments. (C) Invasion ability assay of NCM460 cells in the presence of different glucose concentrations, using Transwell (200×). (D) Number of migrated cells in (C). (E) Tumors in the different non-diabetic and diabetic mouse groups. (F) Suppression rate in each group in (E). The data are presented as mean ± SD, * p < 0.05 compared with the LF or Non-diabetic NT5DC3 group, # p < 0.05 compared with the normal group in (D), # p < 0.05 compared with the normal group in (F). (A–D) the experiments were repeated three times (n = 3); (E,F) the experiments were repeated five times (n = 5).
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